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. 2020 Apr 28;9(5):1087. doi: 10.3390/cells9051087

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mad2 and Mps1 are not recruited to unattached kinetochores in 2-cell P. mammillata embryos. (a) Representative images of live eggs (top, middle rows) and 2-cell embryos (bottom row) expressing histone H2B-Venus and Mad2-Tomato. (b) Ratio of Mad2-Tomato fluorescence intensity in chromosome region (A) and cytoplasm (B) in nocodazole-treated eggs and 2-cell embryos (c) Representative images of live eggs (top) and 2-cell embryos (bottom) expressing histone H2-Venus and Mps1-Tomato. (d) Ratio of Mps1-Tomato fluorescence intensity in chromosome region (A) and cytoplasm (B) in nocodazole-treated eggs and 2-cell embryos. White squares in (a,c) indicate chromatin-containing regions enlarged (5×) on the right images. Scale bar = 30 µm.