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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
siRNA mediated depletion of klotho in HT-22 hippocampal neuronal cells (A) Western Blot analysis of klotho membrane and secreted forms expression after transfection; (B) representative Western Blot. Bars indicate SD, n = 3, ** p < 0.01 (one-way ANOVA and Dunnett’s a posteriori test).