Figure 2.

A549 cells transfected with CRISPR-Cas9 phage plasmid. Cells were stained with anti-Cas9 antibody (red), phalloidin-Alexafluoro-488 (green) to stain the F-actin, and 4′,6-diamidino-2-phenylindole (DAPI; blue). Non-transfected cells and cells incubated with the secondary antibody alone were included as negative controls. Images were taken under a confocal fluorescence microscope. Scale bar, 100 µm.