Table 4.
Parameters of the UPLC-MS/MS system to measure TTX and analogs.
| Analyte | Retention Time(min) | Precursor Ion(m/z) | Product Ion(m/z) | Cone Voltage (V) |
Collision Energy(eV) |
|---|---|---|---|---|---|
| TTX | 2.77 | 320.0 | 302.0 * 161.8 |
45 | 25 35 |
| 5-deoxy TTX/ 11-deoxy TTX |
2.61 | 304.5 | 286.29 162.10 |
40 | 25 20 |
| 4,9-anhydro TTX | 2.61 | 302.20 | 256.13 161.83 |
40 | 35 25 |
| 6,11-dideoxy TTX | 2.51 | 288.54 | 270.11 224.69 |
40 | 25 25 |
| 5,6,11-trideoxy TTX | 2.22 | 271.90 | 254.11 162.07 |
40 | 25 20 |
| anhydro-5,6,11-trideoxy TTX | 1.85 | 254.02 | 161.98 | 40 | 25 |
*: Quantitative ion.