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. 2020 May 21;9(5):1284. doi: 10.3390/cells9051284

Figure 6.

Figure 6

Differences between mature and hypertrophic-like adipocytes occur in cell size, lipid droplets, IL-6 secretion, and gene expression. (A) Violin plot showing cell area of mature (MAs) and hypertrophic-like (HAs) adipocytes (n = 60). *** p val ≤ 0.001. (B) Representative confocal microscopy images of hMSCs differentiated in MAs and HAs stained with DAPI (nuclei, blue), Bodipy 495/503 (lipid droplets, green) and WGA 632/647 (cell membranes, red; scale bars, 200 µm). (C) Violin plot showing lipid area/cell measured by 3D analysis on 2973 LDs (from 214 MAs) and on 1168 LDs (from 206 HAs). *** p val ≤ 0.001. (D) Schematic representation of 3D analysis results from LDs in MAs and HAs (i.e., mean of number of LD/cell, LD area, and total LD area/cell), as described in B. (E) Bar graph reporting IL-6 concentration (pg/mL) determined by ELISA on culture supernatant of MAs and HAs. Data are reported as mean ±SEM from three independent experiments. *** p val ≤ 0.001. (F) Relative PPARGΔ5/cPPARG mRNA levels (qPCR) in MAs and HAs. MAs were used as reference sample and PPIA as reference gene. Data are reported as mean ±SEM from three independent experiments. *** p val ≤ 0.001. (G) Relative mRNA quantifications (signed fold-changes) in HAs vs. MAs (dotted line). PPIA was used as reference gene. Data are reported as mean ±SEM from three independent experiments. * p val ≤ 0.05, ** p val ≤ 0.01 and *** p val ≤ 0.001.