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. 2020 May 1;9(5):1126. doi: 10.3390/cells9051126

Figure 1.

Figure 1

Quantification of CAD knockdown. (A) Analysis of CAD knockdown. 293T cells were transfected with siRNAs targeting CAD (CAD-siRNA), or a negative control (ctrl siRNA). The cells were harvested 48 h post-transfection and the lysates were subjected to SDS-PAGE and Western blotting. (B) Quantification of CAD knockdown. The Western blot signals for CAD knockdown (as shown in Figure 1A) were measured and normalized to the GAPDH signals. The negative control (ctrl siRNA) was set to 100% and the efficiency of CAD knockdown was calculated (**** p ≤ 0.0001).