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. 2020 May 1;9(5):1126. doi: 10.3390/cells9051126

Figure 4.

Figure 4

Recruitment of CAD into NP-induced inclusion bodies. (A) Colocalization between CAD and NP-induced inclusion bodies. Huh7 cells were transfected with plasmids encoding FLAG/HA-CAD and EBOV-NP as indicated. 48 h post-transfection, the cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. FLAG-tagged CAD (shown in green) was detected using an anti-FLAG antibody and NP (shown in red) was stained with anti-EBOV NP antibodies. (B) Recruitment of CAD into inclusion bodies occurs in the presence of VP35. Huh7 cells were transfected with plasmids encoding FLAG/HA-CAD, EBOV-NP, and myc-EBOV-VP35 as indicated. 48 h post-transfection, the cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. FLAG-tagged CAD (shown in green) was detected using an anti-FLAG antibody, NP (shown in red) was stained with anti-EBOV NP antibodies, and myc-tagged VP35 (shown in turquoise) with an anti-myc antibody. The nuclei were stained with DAPI (shown in blue), and the cells were visualized by confocal laser scanning microscopy. The scale bars indicate 10 µm. The arrows point out colocalization, and the insets show magnifications of the indicated areas. Merge shows an overlay of all three channels.