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. 2020 May 20;12(5):563. doi: 10.3390/v12050563

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Binding of gp44 to CI. (A) Superimposed SEC profiles of the untagged CI (orange) and gp44 (gray) proteins, purified by Ulp1 cleavage from the His-SUMO fusion proteins. (B) SEC profile of the CI–gp44 mixture, showing that no new peaks corresponding to a CI–gp44 complex appeared. (C,D) SDS-PAGE of eluates from affinity pulldowns of untagged CI with His-SUMO-gp44 (C), and untagged gp44 with His-SUMO-CI (D). M, marker; FT, flow-through. Sequential elutions with 20, 50, and 300 mM imidazole are shown. The bands corresponding to His-SUMO-gp44 (S44), His-SUMO-gp44-NTD (S44N), untagged CI (CI), His-SUMO-CI (SCI), untagged gp44 (44), and untagged gp44-NTD (44N) are indicated.