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Nuclease protection assay. Agarose gel electrophoresis of 21 nM cI–cro (CC) DNA incubated with gp44 (A) or CI (B) without (left lanes) and with (right lanes) subsequent T5 exonuclease and proteinase K treatment. The -fold molar excess of protein to DNA is shown above the gel. The position of the cI–cro DNA is indicated (CC).
