Table 2.
Infecting Phage | Recipient Strain ¶ | Complementing Protein | Residues | IPTG | Phage Titer (PFU/mL) |
---|---|---|---|---|---|
WT | KM12 | gp44 Full Length | 1–331 | – | 1010 |
Δ44 | KM12 | gp44 Full Length | 1–331 | – | N.D. § |
Δ44 | KM12 | gp44 Full Length | 1–331 | + | 108 |
Δ44 | KM44 | gp44-NTD | 1–271 | + | N.D. |
Δ44 | KM45 | gp44-CTD | 272–331 | + | N.D. |
Δ44 | KM57 | gp44-NTD-Long | 1–292 | + | N.D. |
Δ44 | KM59 | gp44-CTD-Long | 33–331 | + | N.D. |
Δ44 | KM55 | gp44-NTD-Long+ | 1–323 | + | 108 |
Δ44 | KM54 | gp44-CTD-Long+ | 8–331 | + | 108 |
¶ See Table 1. § N.D. = not detected; no plaques were observed upon plating undiluted lysate.