Figure 4.

3-BP induces reactive oxygen species (ROS) formation. (A, B) 3-BP0000000 treatment results in generation of ROS. A logarithmically growing culture of wild type cells pre-loaded with dihydroethidium (A) or dihydrorhodamine 123 (B) was treated with indicated concentrations of 3-BP for 2 h. Fluorescence was detected using a fluorescence microscope and quantified with a fluorescence plate reader. Error bars represent mean value ± standard deviations of mean (n = 3). Average fluorescence value for untreated cultures was set to 1.