Figure 5.

Homologous recombination is crucial for tolerance to 3-BP: (A,B) Homologous recombination, nonhomologous end-joining and base excision repair pathways are required for maintaining viability of yeast cells in the presence of 3-BP. Serial dilutions of indicated strains were plated on solid media in the presence or absence of 3-BP at 30 °C and photographed after 3 days. (C–E) 3-BP treatment triggers formation of homologous recombination repair centers. Logarithmically growing cultures of cells bearing Mre11-YFP (A), Rfa1-YFP (B) and Rad52-YFP (C) were treated with 3-BP or MMS for 1.5 h. Next, cells were washed and processed for microscopic analysis. Error bars represent mean value ± standard deviations of mean (n = 3).