Figure 2.

APCs derived from HSPCs stimulated with Pam₃CSK₄ or depleted zymosan prime enhanced Th1 and Th17 responses in OVA-specific CD4 T cell cocultures. APCs obtained as shown in Figure 1 were loaded with the OVA_323–339 peptide and cocultured with CFSE-labeled CD4 T cells isolated from OT-II mice at 1:5 ratio (APC:T cell). Following three days of coculture, T cells were harvested for (A) flow cytometry analysis of proliferating CD4⁺ T cells and (B) cytokine production (IL-17A and IFN-γ) after 24 h of restimulation with PMA and ionomycin. Cytokine data was normalized by CD4 T cell numbers (shown in Figure A1E) and represent means ± SD of triplicate cultures, * p < 0.05, ** p < 0.01, *** p < 0.001 with respect to day 0 none. Results shown are from one experiment representing three independent experiments.