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. 2020 May 11;12(5):530. doi: 10.3390/v12050530

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Phylogenetic analysis of nucleotide sequence alignment of whole genome sequences of AstV representative members retrieved from GenBank and the BoAstV PE3373/2019/Italy detected in this study (identified with a red circle). Strains associated with encephalitis are identified with a black circle. Accession numbers are provided in brackets. Bar indicates the estimated numbers of nt substitutions per site. Bootstrap values ≥70 are shown. (b) Phylogenetic analysis of amino acid sequence alignment of the ORF 2 coding region of AstV representative members retrieved from GenBank and the BoAstV PE3373/2019/Italy detected in this study (identified with a red circle). Strains associated with encephalitis are identified with a black circle. Accession numbers are provided in brackets. The bar indicates the estimated numbers of aa substitutions per site. Bootstrap values ≥70 are shown. Sequences of both trees were retrieved from a previously published study [23].

(a) Phylogenetic analysis of nucleotide sequence alignment of whole genome sequences of AstV representative members retrieved from GenBank and the BoAstV PE3373/2019/Italy detected in this study (identified with a red circle). Strains associated with encephalitis are identified with a black circle. Accession numbers are provided in brackets. Bar indicates the estimated numbers of nt substitutions per site. Bootstrap values ≥70 are shown. (b) Phylogenetic analysis of amino acid sequence alignment of the ORF 2 coding region of AstV representative members retrieved from GenBank and the BoAstV PE3373/2019/Italy detected in this study (identified with a red circle). Strains associated with encephalitis are identified with a black circle. Accession numbers are provided in brackets. The bar indicates the estimated numbers of aa substitutions per site. Bootstrap values ≥70 are shown. Sequences of both trees were retrieved from a previously published study [23].