Figure 4.

The linker/OB-foldnetwork of interactions is critical to SSB interactome function. Top, a schematic of an SSB-ssDNA complex demonstrating how the linker-OB-fold network of interactions produces a stable complex where the ssDNA is coated and protected. Here, the linker from the first tetramers bind to the OB-folds in adjacent tetramers, while other linkers are exposed and available for partner binding, in this case, RecG (left inset). Here, the helicase is presented as a ribbon diagram (purple) and a linker domain (cyan) is modeled into the OB-fold. When SSB-tetramers interact, some OB-folds are occupied by linker domains (cyan) while others bind ssDNA (purple). For clarity, only two monomers of the tetramer are shown and represented as ribbon diagrams (colored green). The schematic is adapted from [63].