Figure 4.

Averaged calibration curve with 0.2 µg·mL⁻¹ AFB1-BSA conjugate coating and 150 ng·mL⁻¹ mAb with aflatoxin B1 competitor concentrations ranging from 0.006 ng·mL⁻¹ to 500,000 ng·mL⁻¹. Measuring signal was achieved by indirect readout at 405 nm with mouse-specific secondary antibody conjugated to horseradish peroxidase and respective substrate LOD: limit of detection; IC₅₀: half maximal inhibitory concentration. Each data point represents the mean ± SD (n = 12).