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cMID calibration curves with 2 µg·mL−1 AFB1-BSA conjugate coating and 2.5 µg·mL−1 biotinylated mAb in combination with (A) 700 nm streptavidin-functionalized magnetic particles, and (B) 70 nm streptavidin-functionalized magnetic particles with aflatoxin B1 competitor concentrations ranging from 0.006 ng·mL−1 to 500,000 ng·mL−1. LOD, limit of detection; IC50, half maximal inhibitory concentration. Each data point represents the mean ± SD (n = 2).
