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Magnetic immunodetection checkerboard titration determining suitable aflatoxin B1-BSA (AFB1-BSA) coating concentrations and aflatoxin B1-specific biotinylated monoclonal antibody AFB1_002 concentrations. The readout was done by detecting 700 nm streptavidin-functionalized magnetic particles coupled to biotinylated AFB1_002 using FMMD (n = 1).
