Table 4.
Non-exhaustive list of MAP1LC3 antibodies.
| Antibodies | Applications, Conditions 1 | Limitations |
|---|---|---|
| Polyclonal anti-MAP1LC3B antibody (#NB600-1384, Novus Biologicals) | Western blot 1:1000 in 5% (w/v) non-fat milk, 3h, room temperature. For example, ref [84,86,87] |
MAP1LC3A cross-reaction |
| Polyclonal affinity-purified rabbit anti-MAP1LC3B antibody (#2775; Cell signaling Technology) | Western blot, 1:1000 Used with human primary cells (PBMCs, dendritic cells, monocytes, macrophages), cell lines. For example ref [12] |
|
| Rabbit anti-MAP1LC3B antibody (#3868,Cell Signaling) | Immunofluorescence 1:200 in PBS-1% (w/v) BSA. | |
| Anti-MAP1LC3B antibody (#M186-3, MBL Inc.) | Western blot 0.5 µg/mL and immunofluorescence 5 µg/mL in TBS containing 1% non-fat milk. Used for example with sciatic nerves sections from Lewis rats; with malignant glioblastoma-derived U-251MG cells; with mouse spleen and salivary glands cells; with colonic cells from mice with colitis (Figure 4). For example, ref. [29,30,57] |
|
| Monoclonal rabbit anti-MAP1LC3B antibody, clone D11 (#3686, Cell Signaling) | Immunofluorescence. For example ref [84,86,87] |
See the legend of Figure 4B |
| Polyclonal affinity-purified rabbit anti-MAP1LC3A/B antibody (#4108; Cell signaling Technology) | Immunofluorescence 1:200 dilution. Used with cell lines. For example, ref [12] |
1 These conditions are routinely used in our respective laboratories. They have to be adapted to each type of substrate and technique. See abbreviations in the abbreviations section.