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. 2020 May 16;9(5):1238. doi: 10.3390/cells9051238

Figure 5.

Figure 5

The neflb splicing variants assembly properties in SW13vim cells. (A), eGFP-neflbE4 (i) and eGFP-neflbE3 (i’) were expressed in SW13vim cells alone or together with the mouse NEFM (ii,ii’, red) or NEFH (iii,iii’, red). The assembly pattern was characterized at 48 h post transfection at 37 °C. (B), eGFP-neflbE3 and NEFM were co-transfected and the bundle formation was observed at 37 °C and 28 °C. Bottom panel, bar graph showing the percentage of cells carrying filaments, bundles or diffuse labeling. At 28 °C, the proportion of cells carrying bundles made of neflb3E and NEFM was less than the proportion of cells carrying bundles at 37 °C. * p < 0.05, (n = 3 cultures, 60 to 100 cells per coverslip) one-way ANOVA Tukey’s HSD post-hoc analysis.