Figure 8.

SG formation in IBV infected cells is correlated with increased translational shut-off. (A) Vero cells were mock infected or infected with IBV. At 23 hpi, positive control cells were treated with sodium arsenite (NaAs), to induce inhibition of translation. At 24 hpi, cells were treated with puromycin followed by emetine. Cells were then fixed, and nascent polypeptides labelled with puromycin were detected using an anti-puromycin antibody (red), stress granules labelled with anti-G3BP1 (green) and IBV infected cells labelled with an anti-IBV antibody (cyan). Nuclei were stained with DAPI (blue). Scale bar indicates 20 µm. (B) Representative scatterplot of de novo protein synthesis from (A), measured by fluorescence intensity of the puromycin signal analyzed in 80 cells. Data presented is representative of three independent replicates. Asterisks indicate statistical significance, as measured by one-way ANOVA, *** p = 0.0004; **** p < 0.0001.