Table 1.
Expression stability values for switchgrass candidate reference genes calculated by BestKeeper.
| Rank | Total | CdL | CdR | PbL | PbR | HgL | HgR | CrL | CrR | AsL | AsR |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | U2AF (2.51 ± 0.61) | UCE (1.09 ± 0.24) | eEF4a (1.16 ± 0.33) | U2AF (1.01 ± 0.24) | UBC (1.10 ± 0.24) | U2AF (1.07 ± 0.25) | U2AF (2.54 ± 0.65) | UBC (1.26 ± 0.27) | FTSH4 (0.72 ± 0.18) | U2AF (1.40 ± 0.34) | eEF4a (0.87 ± 0.26) |
| 2 | eEF4a (2.54 ± 0.74) | FTSH4 (1.28 ± 0.3) | ACT12 (1.37 ± 0.28 | ACT12 (1.10 ± 0.22) | eEF1a (1.14 ± 0.23) | UCE (1.26 ± 0.27) | ACT2 (2.59 ± 0.78) | U2AF (1.29 ± 0.31) | UCE (1.10 ± 0.25) | UBQ2 (1.46 ± 0.28) | ACT2 (1.02 ± 0.31) |
| 3 | ACT12 (2.67 ± 0.55) | U2AF (1.41 ± 0.33) | U2AF (1.42 ± 0.34) | FTSH4 (1.31 ± 0.31) | UCE (1.49 ± 0.32) | FTSH4 (1.34 ± 0.31) | UBC (2.73 ± 0.66) | ACT12 (1.33 ± 0.27) | UBC (1.16 ± 0.27) | UCE (1.65 ± 0.37) | U2AF (1.05 ± 0.25) |
| 4 | FTSH4 (3.24 ± 0.78) | ACT12 (1.59 ± 0.32) | UBQ1 (1.60 ± 0.32) | UBQ1 (1.38 ± 0.29) | eEF4a (1.50 ± 0.43) | ACT12 (1.34 ± 0.28) | UBQ6 (3.00 ± 0.68) | UCE (1.57 ± 0.33) | ACT12 (1.40 ± 0.29) | eEF1a (1.78 ± 0.40) | UBQ2 (1.07 ± 0.20) |
| 5 | UBQ2 (3.41 ± 0.62) | UBC (1.86 ± 0.38) | CYP5 (1.62 ± 0.34) | 18S rRNA (1.45 ± 0.13) | U2AF (1.54 ± 0.37) | UBQ1 (1.41 ± 0.30) | eEF4a (3.27 ± 1.00) | eEF4a (1.97 ± 0.57) | eEF4a (1.55 ± 0.46) | ACT12 (1.84 ± 0.38) | eEF1a (1.20 ± 0.27) |
| 6 | CYP5 (3.59 ± 0.78) | UBQ2 (2.19 ± 0.41) | FTSH4 (1.71 ± 0.40) | UCE (1.53 ± 0.32) | UBQ2 (2.00 ± 0.35) | eEF4a (1.68 ± 0.48) | ACT12 (3.32 ± 0.70) | FTSH4 (2.11 ± 0.49) | U2AF (1.61 ± 0.41) | UBC (1.97 ± 0.43) | CYP5 (1.43 ± 0.32) |
| 7 | UCE (3.82 ± 0.83) | eEF4a (2.23 ± 0.65) | eEF1a (1.82 ± 0.38) | eEF4a (1.66 ± 0.47) | UBQ1 (2.07 ± 0.41) | UBC (1.89 ± 0.40) | UCE (3.96 ± 0.89) | CYP5 (3.25 ± 0.70) | eEF1a (1.89 ± 0.41) | eEF4a (2.11 ± 0.62) | FTSH4 (1.46 ± 0.35) |
| 8 | UBQ1 (3.87 ± 0.82) | CYP5 (2.60 ± 0.57) | UCE (2.12 ± 0.45) | CYP5 (1.68 ± 0.36) | CYP5 (2.18 ± 0.45) | UBQ2 (2.06 ± 0.39) | FTSH4 (4.04 ± 1.08) | UBQ2 (3.27 ± 0.60) | ACT2 (2.27 ± 0.71) | FTSH4 (2.22 ± 0.53) | UBQ1 (1.61 ± 0.35) |
| 9 | eEF1a (4.12 ± 0.88) | UBQ1 (2.87 ± 0.62) | UBC (2.18 ± 0.48) | UBC (1.91 ± 0.40) | FTSH4 (2.28 ± 0.55) | UBQ6 (2.10 ± 0.46) | CYP5 (4.13 ± 0.96) | UBQ6 (3.91 ± 0.89) | CYP5 (2.63 ± 0.60) | CYP5 (2.57 ± 0.55) | UBQ6 (1.74 ± 0.38) |
| 10 | UBC (4.28 ± 0.93) | eEF1a (3.13 ± 0.66) | UBQ2 (2.48 ± 0.42) | UBQ2 (2.17 ± 0.40) | ACT2 (2.69 ± 0.74) | CYP5 (2.29 ± 0.49) | UBQ2 (4.18 ± 0.77) | UBQ1 (3.98 ± 0.85) | UBQ6 (2.96 ± 0.65) | UBQ1 (2.60 ± 0.57) | UCE (2.01 ± 0.48) |
| 11 | ACT2 (4.35 ± 1.29) | ACT2 (3.3 ± 0.98) | ACT2 (2.78 ± 0.82) | eEF1a (2.59 ± 0.54) | UBQ6 (2.91 ± 0.58) | 18S rRNA (2.63 ± 0.25) | eEF1a (4.26 ± 0.99) | eEF1a (4.36 ± 0.92) | UBQ1 (2.98 ± 0.66) | UBQ6 (3.43 ± 0.76) | ACT12 (2.23 ± 0.45) |
| 12 | UBQ6 (4.38 ± 0.95) | 18S rRNA (3.86 ± 0.38) | UBQ6 (3.06 ± 0.62) | UBQ6 (2.96 ± 0.65) | CYP2 (3.72 ± 0.91) | eEF1a (2.96 ± 0.62) | UBQ1 (4.76 ± 1.06) | 18S rRNA (4.93 ± 0.48) | UBQ2 (3.05 ± 0.54) | 18S rRNA (3.97 ± 0.42) | UBC (2.57 ± 0.54) |
| 13 | 18S rRNA (7.61 ± 0.78) | UBQ6 (4.29 ± 0.95) | 18S rRNA (4.75 ± 0.51) | ACT2 (3.35 ± 0.96) | ACT12 (4.43 ± 0.88) | ACT2 (4.01 ± 1.20) | CYP2 (5.01 ± 1.34) | ACT2 (5.38 ± 1.57) | CYP2 (3.89 ± 1.00) | ACT2 (4.78 ± 1.44) | 18S rRNA (3.76 ± 0.42) |
| 14 | CYP2 (11.27 ± 3.11) | CYP2 (6.36 ± 1.91) | CYP2 (5.32 ± 1.33) | CYP2 (10.46 ± 3.09) | 18S rRNA (7.41 ± 0.75) | CYP2 (14.04± 3.97) | 18S rRNA (9.21 ± 1.05) | CYP2 (10.69 ± 3.06) | 18S rRNA (5.76 ± 0.65) | CYP2 (8.34 ± 2.50) | CYP2 (6.52 ± 1.75) |