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. 2020 May 3;11(5):502. doi: 10.3390/genes11050502

Table 2.

Expression stability values for switchgrass candidate reference genes calculated by NormFinder.

Rank Total CdL CdR PbL PbR HgL HgR CrL CrR AsL AsR
1 U2AF (0.463) U2AF (0.095) CYP5 (0.138) 18S rRNA (0.105) eEF1a (0.175) ACT12 (0.130) U2AF (0.236) ACT12 (0.236) U2AF (0.081) UBQ2 (0.192) U2AF (0.079)
2 CYP5 (0.484) ACT12 (0.269) FTSH4 (0.182) ACT12 (0.224) UCE (0.190) CYP5 (0.159) UBC (0.247) UBC (0.313) UCE (0.179) ACT12 (0.302) CYP5 (0.138)
3 UBQ1 (0.539) UBQ1 (0.279) U2AF (0.184) U2AF (0.229) UBC (0.247) UBQ1 (0.212) UBQ2 (0.251) U2AF (0.369) eEF4a (0.294) U2AF (0.326) UBQ6 (0.273)
4 eEF4a (0.569) 18S rRNA (0.384) UBQ1 (0.201) FTSH4 (0.276) U2AF (0.344) UCE (0.274) UCE (0.258) UCE (0.375) FTSH4 (0.299) 18S rRNA (0.346) UBQ2 (0.275)
5 eEF1a (0.603) UCE (0.414) UBQ2 (0.241) UCE (0.279) UBQ1 (0.364) UBQ6 (0.293) eEF1a (0.329) FTSH4 (0.548) eEF1a (0.311) FTSH4 (0.398) eEF4a (0.302)
6 UCE (0.728) FTSH4 (0.426) UCE (0.288) UBC (0.309) UBQ2 (0.483) UBQ2 (0.307) CYP5 (0.349) 18S rRNA (0.568) CYP5 (0.315) UBQ1 (0.403) UCE (0.345)
7 UBQ2 (0.748) CYP5 (0.437) UBC (0.305) UBQ2 (0.316) CYP5 (0.513) U2AF (0.320) 18S rRNA (0.386) eEF4a (0.655) UBC (0.366) eEF1a (0.425) UBQ1 (0.347)
8 ACT12 (0.757) eEF1a (0.451) UBQ6 (0.397) UBQ1 (0.346) eEF4a (0.546) 18S rRNA (0.346) eEF4a (0.428) UBQ2 (0.743) UBQ2 (0.390) UBC (0.476) UBC (0.359)
9 18S rRNA (0.812) UBQ2 (0.494) eEF1a (0.404) CYP5 (0.420) UBQ6 (0.633) eEF1a (0.443) UBQ1 (0.462) CYP5 (0.851) UBQ1 (0.503) CYP5 (0.509) eEF1a (0.370)
10 FTSH4 (0.869) eEF4a (0.539) ACT12 (0.535) eEF4a (0.548) FTSH4 (0.686) eEF4a (0.510) UBQ6 (0.625) UBQ6 (1.084) UBQ6 (0.509) UCE (0.596) ACT12 (0.520)
11 UBQ6 (0.903) UBC (0.586) eEF4a (0.557) eEF1a (0.555) ACT2 (0.832) FTSH4 (0.576) FTSH4 (0.653) UBQ1 (1.162) ACT12 (0.521) eEF4a (0.642) ACT2 (0.541)
12 UBC (1.038) UBQ6 (1.082) 18S rRNA (0.716) UBQ6 (0.699) 18S rRNA (0.877) UBC (0.627) CYP2 (0.972) eEF1a (1.247) 18S rRNA (0.601) UBQ6 (0.787) FTSH4 (0.622)
13 ACT2 (1.467) ACT2 (1.273) ACT2 (1.016) ACT2 (1.249) CYP2 (1.566) ACT2 (1.615) ACT12 (1.338) ACT2 (1.840) ACT2 (1.093) ACT2 (1.825) 18S rRNA (0.732)
14 CYP2 (3.391) CYP2 (2.651) CYP2 (1.825) CYP2 (3.522) ACT12 (1.580) CYP2 (4.373) ACT2 (1.456) CYP2 (3.497) CYP2 (1.147) CYP2 (3.037) CYP2 (2.221)