Table 3.
The most stable and least stable combination of reference genes based on RefFinder analysis.
| Method | Stability (High-Low) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |
| Cd L | ||||||||||||||
| BestKeeper | UCE | FTSH4 | ACT12 | U2AF | 18Sr | UBC | UBQ2 | CYP5 | UBQ1 | eEF4a | eEF1a | UBQ6 | ACT2 | CYP2 |
| NormFinder | U2AF | ACT12 | UBQ1 | 18Sr | UCE | FTSH4 | CYP5 | eEF1a | UBQ2 | eEF4a | UBC | UBQ6 | ACT2 | CYP2 |
| Genorm | U2AF | ACT12 | UCE | FTSH4 | UBC | UBQ2 | 18Sr | CYP5 | UBQ1 | eEF1a | eEF4a | UBQ6 | ACT2 | CYP2 |
| RefFinder | U2AF | ACT12 | UCE | FTSH4 | 18Sr | UBQ1 | UBQ2 | CYP5 | UBC | eEF1a | eEF4a | UBQ6 | ACT2 | CYP2 |
| Pb L | ||||||||||||||
| BestKeeper | 18Sr | ACT12 | U2AF | UBQ1 | FTSH4 | UCE | CYP5 | UBC | UBQ2 | eEF4a | eEF1a | UBQ6 | ACT2 | CYP2 |
| NormFinder | 18Sr | ACT12 | U2AF | FTSH4 | UCE | UBC | UBQ2 | UBQ1 | CYP5 | eEF4a | eEF1a | UBQ6 | ACT2 | CYP2 |
| Genorm | 18Sr | ACT12 | FTSH4 | U2AF | UCE | CYP5 | UBQ1 | UBC | UBQ2 | eEF1a | eEF4a | UBQ6 | ACT2 | CYP2 |
| RefFinder | 18Sr | ACT12 | U2AF | FTSH4 | UCE | UBQ1 | CYP5 | UBC | UBQ2 | eEF4a | eEF1a | UBQ6 | ACT2 | CYP2 |
| Hg L | ||||||||||||||
| BestKeeper | 18Sr | U2AF | UCE | ACT12 | UBQ1 | FTSH4 | UBQ2 | UBC | UBQ6 | eEF4a | CYP5 | eEF1a | ACT2 | CYP2 |
| NormFinder | ACT12 | CYP5 | UBQ1 | UCE | UBQ6 | UBQ2 | U2AF | 18Sr | eEF1a | eEF4a | FTSH4 | UBC | ACT2 | CYP2 |
| Genorm | UBQ6 | CYP5 | U2AF | ACT12 | UCE | 18Sr | UBQ1 | eEF4a | UBQ2 | eEF1a | FTSH4 | UBC | ACT2 | CYP2 |
| RefFinder | ACT12 | U2AF | CYP5 | UBQ6 | UCE | UBQ1 | 18Sr | UBQ2 | eEF4a | FTSH4 | eEF1a | UBC | ACT2 | CYP2 |
| Cr L | ||||||||||||||
| BestKeeper | UBC | ACT12 | U2AF | UCE | 18Sr | FTSH4 | eEF4a | UBQ2 | CYP5 | UBQ1 | UBQ6 | eEF1a | ACT2 | CYP2 |
| NormFinder | ACT12 | UBC | U2AF | UCE | FTSH4 | 18Sr | eEF4a | UBQ2 | CYP5 | UBQ6 | UBQ1 | eEF1a | ACT2 | CYP2 |
| Genorm | UCE | UBC | U2AF | ACT12 | FTSH4 | 18Sr | UBQ2 | eEF4a | CYP5 | UBQ1 | eEF1a | UBQ6 | ACT2 | CYP2 |
| RefFinder | UBC | U2AF | ACT12 | UCE | FTSH4 | 18Sr | eEF4a | UBQ2 | CYP5 | UBQ1 | UBQ6 | eEF1a | ACT2 | CYP2 |
| As L | ||||||||||||||
| BestKeeper | UBQ2 | U2AF | UCE | ACT12 | eEF1a | 18Sr | UBC | FTSH4 | CYP5 | UBQ1 | eEF4a | UBQ6 | ACT2 | CYP2 |
| NormFinder | UBQ2 | ACT12 | U2AF | 18Sr | FTSH4 | UBQ1 | eEF1a | UBC | CYP5 | UCE | eEF4a | UBQ6 | ACT2 | CYP2 |
| Genorm | U2AF | ACT12 | UBQ2 | 18Sr | CYP5 | eEF1a | UBQ1 | FTSH4 | eEF4a | UCE | UBC | UBQ6 | ACT2 | CYP2 |
| RefFinder | UBQ2 | U2AF | ACT12 | 18Sr | eEF1a | UBQ1 | FTSH4 | CYP5 | UCE | UBC | eEF4a | UBQ6 | ACT2 | CYP2 |
| Cd R | ||||||||||||||
| BestKeeper | ACT12 | UBQ1 | eEF4a | U2AF | CYP5 | eEF1a | FTSH4 | UBQ2 | UCE | UBC | 18Sr | UBQ6 | ACT2 | CYP2 |
| NormFinder | CYP5 | FTSH4 | U2AF | UBQ1 | UBQ2 | UCE | UBC | UBQ6 | eEF1a | ACT12 | eEF4a | 18Sr | ACT2 | CYP2 |
| Genorm | UBQ1 | FTSH4 | CYP5 | UBQ2 | U2AF | UCE | UBC | UBQ6 | eEF1a | eEF4a | ACT12 | 18Sr | ACT2 | CYP2 |
| RefFinder | CYP5 | UBQ1 | FTSH4 | U2AF | UBQ2 | ACT12 | UCE | UBC | eEF4a | eEF1a | UBQ6 | 18Sr | ACT2 | CYP2 |
| Pb R | ||||||||||||||
| BestKeeper | eEF1a | UBC | UCE | UBQ2 | U2AF | UBQ1 | eEF4a | CYP5 | FTSH4 | UBQ6 | ACT2 | 18Sr | ACT12 | CYP2 |
| NormFinder | eEF1a | UCE | UBC | U2AF | UBQ1 | UBQ2 | CYP5 | eEF4a | UBQ6 | FTSH4 | ACT2 | 18Sr | CYP2 | ACT12 |
| Genorm | eEF1a | U2AF | UCE | UBQ1 | CYP5 | UBC | UBQ6 | UBQ2 | eEF4a | FTSH4 | 18Sr | ACT2 | CYP2 | ACT12 |
| RefFinder | eEF1a | U2AF | UCE | UBC | UBQ1 | UBQ2 | CYP5 | eEF4a | UBQ6 | FTSH4 | 18Sr | ACT2 | CYP2 | ACT12 |
| Hg R | ||||||||||||||
| BestKeeper | U2AF | UBC | UBQ6 | ACT12 | UBQ2 | ACT2 | UCE | CYP5 | eEF1a | eEF4a | 18Sr | UBQ1 | FTSH4 | CYP2 |
| NormFinder | U2AF | UBC | UBQ2 | UCE | eEF1a | CYP5 | 18Sr | eEF4a | UBQ1 | UBQ6 | FTSH4 | CYP2 | ACT12 | ACT2 |
| Genorm | UBQ1 | UCE | eEF1a | eEF4a | CYP5 | 18Sr | UBC | U2AF | UBQ2 | FTSH4 | UBQ6 | CYP2 | ACT12 | ACT2 |
| RefFinder | UCE | U2AF | UBC | eEF1a | CYP5 | UBQ2 | UBQ1 | eEF4a | 18Sr | UBQ6 | ACT12 | FTSH4 | ACT2 | CYP2 |
| Cr R | ||||||||||||||
| BestKeeper | FTSH4 | UCE | UBC | ACT12 | U2AF | eEF1a | eEF4a | UBQ2 | CYP5 | 18Sr | UBQ6 | UBQ1 | ACT2 | CYP2 |
| NormFinder | U2AF | UCE | eEF4a | FTSH4 | eEF1a | CYP5 | UBC | UBQ2 | UBQ1 | UBQ6 | ACT12 | 18Sr | ACT2 | CYP2 |
| Genorm | UCE | UBC | FTSH4 | U2AF | eEF1a | CYP5 | eEF4a | UBQ2 | UBQ6 | UBQ1 | ACT12 | 18Sr | ACT2 | CYP2 |
| RefFinder | UCE | U2AF | FTSH4 | UBC | eEF4a | eEF1a | CYP5 | UBQ2 | ACT12 | UBQ1 | UBQ6 | 18Sr | ACT2 | CYP2 |
| As R | ||||||||||||||
| BestKeeper | UBQ2 | U2AF | eEF4a | eEF1a | ACT2 | CYP5 | UBQ1 | FTSH4 | UBQ6 | 18Sr | ACT12 | UCE | UBC | CYP2 |
| NormFinder | U2AF | CYP5 | UBQ6 | UBQ2 | eEF4a | UCE | UBQ1 | UBC | eEF1a | ACT12 | ACT2 | FTSH4 | 18Sr | CYP2 |
| Genorm | eEF1a | eEF4a | UBQ1 | UBQ6 | CYP5 | U2AF | UBQ2 | ACT12 | UBC | UCE | ACT2 | FTSH4 | 18Sr | CYP2 |
| RefFinder | U2AF | eEF4a | UBQ2 | eEF1a | CYP5 | UBQ6 | UBQ1 | UCE | ACT2 | UBC | ACT12 | FTSH4 | 18Sr | CYP2 |
| Total | ||||||||||||||
| BestKeeper | ACT12 | U2AF | UBQ2 | eEF4a | FTSH4 | CYP5 | 18Sr | UBQ1 | UCE | eEF1a | UBC | UBQ6 | ACT2 | CYP2 |
| NormFinder | U2AF | CYP5 | UBQ1 | eEF4a | eEF1a | UCE | UBQ2 | ACT12 | 18Sr | FTSH4 | UBQ6 | UBC | ACT2 | CYP2 |
| Genorm | eEF4a | U2AF | CYP5 | 18Sr | FTSH4 | eEF1a | UCE | UBQ1 | UBC | ACT12 | UBQ2 | UBQ6 | ACT2 | CYP2 |
| RefFinder | U2AF | eEF4a | CYP5 | ACT12 | UBQ1 | eEF1a | 18Sr | FTSH4 | UCE | UBQ2 | UBC | UBQ6 | ACT2 | CYP2 |