Skip to main content
. 2020 Apr 29;9(5):1103. doi: 10.3390/cells9051103

Figure 1.

Figure 1

Early phenotypic changes in XX Wnt4 mutants are independent of SOX9 function. (A–D) Immunodetection of the pre-granulosa cell marker FOXL2 (green) in E12.5 XX gonads of Control (A), Sox9cKO (B), Wnt4KO (C) and Sox9cKO Wnt4KO (D) genotypes. (E–H) Immunodetection of the steroidogenic enzyme HSD3B (red) in E12.5 XX gonads of Control (E), Sox9cKO (F), Wnt4KO (G) and Sox9cKO Wnt4KO (H) genotypes. (I–N) RT-quantitative PCR analysis of Foxl2 (I), Axin2 (J), Follistatin (Fst) (K), Sox8 (L), Fgf9 (M) and Amh (N) expression in E12.5 gonads of XX Control, XY Control, XX Sox9cKO, XX Wnt4KO, and XX Sox9cKO Wnt4KO genotypes (N = 3–4 embryos for each genotype). Expression level in XX controls is 1. Graphs show individual values (dots), and the mean fold-change (bars) ± SEM. Nuclei labeled with DAPI are shown in blue. Magnification is the same in all panels. Scale bar = 100 μm. Gonads are outlined with broken white lines. White arrow in G indicates the coelomic vessel.