Table A1.
Cell culture supplements.
| Supplement | Supplier | Stock Solution |
|---|---|---|
| Y-27632 (Y) | Bertin Pharma #T1725 | 10 mM in H2O |
| bFGF | Gibco #13256029 | 5 µg/mL in 10 mM Tris, pH 7.6 with 0.1% BSA |
| Dorsomorphin (DM) | abcam #ab120843 | 10 mM in DMSO |
| CHIR99021 (CHIR) | Axon MedChem #1386 | 6 mM in DMSO |
| Valproic acid (VPA) | Sigma #P6273 | 10 mM in DMEM/F12 |
| SB431542 (SB) | Stemcell #72232 | 40 mM in DMSO |
| L-Ascorbic acid (AA) | Sigma #A4544 | 150 mM in H2O |
| TGFß3 | Sigma #SRP3171 | 10 µg/mL in 5 mM citric acid with 0.1% BSA |
| DMH1 | Bertin Pharma, #16679 | 10 mM in DMF |
| Purmorphamine (PMA) | Stemcell #72202 | 10 mM in DMSO |
| Compound E (CE) | Bertin Pharma, #15579 | 10 mM in DMSO |
| GDNF | Peprotech, #450-10 | 10 µg/mL in 0.1% BSA |
| BDNF | Peprotech, #450-02 | 10 µg/mL in 0.1% BSA |
| CNTF | Peprotech, #450-13 | 10 µg/mL in 0.1% BSA |
| SAG | TargetMol #T1779 | 2.5 mM in DMSO |
| DAPT | Cayman Chemicals #Cay13197-5 | 10 mM in DMSO |
| dbcAMP (AMP) | Sigma #D0627 | 100 mM in H2O |
| Retinoic acid (RA) | Sigma #R2625 | 10 mM in DMSO, protect from light |