Table A3.
Primary antibodies used in this study.
| Antibody | Host and Type | Source | Dilution |
|---|---|---|---|
| OCT4 | Mouse monoclonal | Santa Cruz #sc-5279 | 1:50 |
| SOX2 | Rabbit monoclonal | Cell Signaling #3579 | 1:200 |
| OLIG2 | Rabbit polyclonal | Merck/Millipore #AB9610 | 1:250 |
| NKX6.1 | Mouse monoclonal | DSHB #F55A10 | 5 µg/mL |
| TUJ1 | Rabbit polyclonal | Sigma #T2200 | 1:100 |
| TUJ1 | Mouse monoclonal | Covance #MMS-435P | 1:750 |
| ISLET1 | Rabbit polyclonal | Abcam #ab20670 | 1:125 |
| CHAT | Goat polyclonal | Millipore #AB144P | 1:50 |
| SNAP25 | Rabbit polyclonal | Abcam #41455 | 1:100 |
| VAMP2 | Rabbit monoclonal | Cell signaling #D601A | 1:250 |
| SV2 (SV2A/B/C) | Mouse monoclonal | DSHB #SV2 | 10 µg/mL |
| GT1b | Mouse monoclonal | DSHB #GT1b-1 | 10 µg/µL |
| GD1a | Mouse monoclonal | DSHB #GD1a-1 | 10 µg/mL |
| β-Actin (HRP-coupled) |
Mouse monoclonal | Santa Cruz #sc-47778 | 1:4000 |
GT1b-1 and GD1a were deposited to the Developmental Studies Hybridoma Bank (DSHB) by Schnaar, R.L. SV2 was deposited to the DSHB by Buckley, K.M. NKX6.1 was deposited to the DSHB by Madsen, O.D.