Table 1.
Kd,1M and Zeff values determined by FA salt titration binding assays of RSV Gag∆PR with RSV 167, MΨ-WT and six MΨ mutants.
| RNA | Kd,1M 1 | Zeff 2 | Relative Specificity 3 |
|---|---|---|---|
| RSV 167 | (2.1 ± 0.3) × 100 | 10.1 ± 0.2 | 0.00011 |
| MΨ-WT | (2.3 ± 1.0) × 10−4 | 4.5 ± 0.3 | 1.0 |
| MΨ-A197C | (9.8 ± 4.0) × 10−3 | 6.8 ± 0.4 | 0.023 |
| MΨ-G218C | (7.0 ± 8.8) × 10−1 | 9.1 ± 1.2 | 0.00033 |
| MΨ-AAA | (3.5 ± 4.1) × 10−3 | 5.9 ± 0.8 | 0.066 |
| MΨ-AACA | (4.5 ± 1.1) × 10−4 | 5.0 ± 0.3 | 0.51 |
| MΨ-∆L3 | (3.1 ± 2.9) × 10−2 | 6.3 ± 0.6 | 0.0074 |
| MΨ-∆SL-B | (9.6 ± 8.5) × 10−5 | 3.1 ± 0.6 | 2.4 |
1 Kd,1M reflects the specific, non-electrostatic binding component. 2 Zeff represents the effective charge of a protein–RNA interaction. 3 Relative binding specificity of the RNAs was calculated by Kd,1M(MΨ-WT)/ Kd,1M(mutant) with the MΨ-WT value set to 1.0.