Table 4.
Extrapolation, taking the Newly Developed One Real-Time Polymerase Chain Reaction (NDO-RT-PCR) as a gold standard. Positive, if at least one PCR run (triplicate) was positive. PPV: positive predictive value, NPV: negative predictive value, FNR: false negative rate, FPR: false positive rate. NDO-PCR shows best sensitivity and specificities values compared to the other PCRs.
| 352 Samples (87 Sequenced, 265 not Sequenced), (total Number of Test Runs: 1056) | PCRs | |||
|---|---|---|---|---|
| kDNA | 18S rRNA | TCZ | NDO | |
| True positive | 198 | 3 | 57 | 208 |
| False positive | 134 | 1 | 4 | 0 |
| False negative | 27 | 222 | 168 | 17 |
| True negative | 697 | 830 | 827 | 831 |
| Sensitivity (%) | 88.00 | 1.33 | 25.33 | 92.44 |
| Specificity (%) | 83.87 | 99.88 | 99.52 | 100 |
| PPV (%) | 59.64 | 75.00 | 93.44 | 100 |
| NPV (%) | 96.27 | 78.90 | 83.12 | 98.00 |
| FNR (%) | 12.00 | 98.67 | 74.67 | 7.56 |
| FPR (%) | 16.13 | 0.12 | 0.48 | 0 |
| Accuracy (%) | 84.75 | 78.88 | 83.71 | 98.39 |