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. 2020 May 19;9(5):1253. doi: 10.3390/cells9051253

Figure 3.

Figure 3

Knockdown of Akt1 or p110α increases apoptosis and decreases proliferation in MDA-MB-231 cells. MDA-MB-231 cells were transfected with 100 nM of siRNA to Akt1, Akt2, p110α, p110β, p85α, or p85β. Expression of Akt1, Akt2, p110α, p110β, p85α, p85β, cyclin D1, and cleaved PARP was determined with Western blot (A) 72 h and (B) 96 h after transfection. (C) MDA-MB-231 cells were transfected with siRNA to AMPKα1, AMPKα2, Akt1, or p110α alone or in combinations. Transfection concentrations were (1) individual siRNA: 50 nM, (2) combination siRNA: 50 nM each (100 nM total), and (3) siNTC: 100 nM. Expression of AMPKα1, AMPKα2, Akt1, p110α, and cleaved PARP was determined with Western blot 72 h after transfection. (D) MDA-MB-231 cells were transfected with siRNA to AMPKα1, AMPKα2, Akt1, or p110α alone or in combinations. Transfection concentrations were (1) individual siRNA: 50 nM, (2) combination siRNA: 50 nM each (100 nM total), and (3) siNTC: 100 nM. Cells were plated at a density of 3000 cells/well 48 h after transfection. SRB growth assay was performed 48 h after plating (n = 12). *indicates p-value < 0.0001; NTC, Non-targeting control was used as a negative control.