Fig. 2.

Effect of resveratrol on Ang II-mediated induction of hypertrophic markers in RL-14 and H9c2 cells. RL-14 (a) and H9c2 (b) cells were treated for 24 h with vehicle, 10 μM Ang II, 10 μM Ang II in combination with (2, 10 or 50 μM resveratrol) or (2, 10 or 50 μM resveratrol alone) in SFM. Total RNA was isolated using TRIzol, the mRNA expression levels of β-MHC/α-MHC and ANP were assessed using real-time PCR and their levels were normalized to β-actin housekeeping gene. The results are presented as the mean and SEM based on at least 3 individual experiments. Data were analyzed using one-way ANOVA followed by Student–Newman–Keuls as post hoc test. ⁺p < 0.05 significantly different from control group. *p < 0.05 significantly different from Ang II-treated group