Fig. 3.

Effect of resveratrol on mRNA expression and protein expression levels of CYP1B1 in RL-14 cells. RL-14 cells were treated for 24 h with vehicle, 10 μM Ang II, 10 μM Ang II in combination with (2, 10 or 50 μM resveratrol) or (2, 10 or 50 μM resveratrol alone) in SFM. CYP1B1 mRNA expression (a) and protein expression levels (b) were determined using real-time PCR and Western blot analysis, respectively. For real-time PCR, total RNA was isolated using TRIzol reagent, the mRNA level was quantified and its level was normalized to β-actin housekeeping gene. For Western blot analysis, protein levels were detected using the enhanced chemiluminescence method. The intensity of protein band was normalized to the signals obtained for β-actin or GAPDH protein and quantified using ImageJ®. The results are presented as the mean and SEM based on at least 3 individual experiments. Data were analyzed using one-way ANOVA followed by Student–Newman–Keuls as post hoc test. ⁺p < 0.05 significantly different from control group. *p < 0.05 significantly different from Ang II-treated group