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. 2020 May 17;9(5):1241. doi: 10.3390/cells9051241

Figure 1.

Figure 1

Osmotic water permeability of human ejaculated semen from normospermic (1) and sub-fertile (2) subjects with (+HPV) and without (−HPV) HPV infection. The osmotic water permeability was measured by exposing sperm cells to a 150 mOsm osmotic gradient and expressed as Pf (see Materials and methods). (A) Bars represent means ± SEM of 4–15 single shots for each of 8–10 different experiments; the individual data points are also shown (bullet point). a, p < 0.05 vs. normospermic HPV-positive, sub-fertile HPV-negative and sub-fertile HPV-positive (Kruskal–Wallis followed by Dunn’s Multiple Comparison Test). (B–E) Representative traces of stopped-flow osmotic water permeability measurements obtained from ejaculated semen of normospermic (B,C) and sub-fertile (D,E) subjects with (C,E) and without (B,D) HPV. K relative values of single curves are also shown.