Figure 2.

Representative immunofluorescence confocal microscopy images of colocalization of AQP8 and HPV in human sperm. (A) Green labeling indicates the presence of HPV, red labeling the expression of AQP8, while nuclei were counterstained by DAPI (blue). Yellow labeling shows colocalization signal of AQP8 with HPV. Scale bar, 5 µm. (B–D) Images show single labeling for HPV (green; B), AQP8 (red; C) and nuclei (DAPI; D). (E) Colocalization graphs, measured in the green line position in panel A, showing the overlap of the fluorescence signals originated by AQP8 and HPV staining.