Figure 5.

Effect of stress conditions on expression of the mbcAT operon in M. tuberculosis. Wild-type H37Rv transformed with plasmid pGMCS-P1-mTurq-P_mbcA-mVenus-mbcA was grown in complete 7H9 medium. At day 0, cells were harvested and resuspended in phosphate saline buffer (PBS) buffer (starvation), or 7H9 medium supplemented with 5 mM H₂O₂ or 0.5 mM DETA/NO. At the indicated times, samples were harvested, fixed with 4% p-formaldehyde and observed by fluorescent microscopy using large field Leica DMIRB, magnification 630×.