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. 2020 May 16;12(5):329. doi: 10.3390/toxins12050329

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Induction of the mbcAT operon expression in M. tuberculosis upon infection of macrophages. Mtb H37Rv/pGMCS-P1-mTurq-P_mbcA-mVenus-mbcA was used to infect human (a) or mouse (b) macrophages at multiplicity of infection of 0.1. Infected cells were harvested at day 0 (D0) or 24 h post-infection (D1), fixed with 4% p-formaldehyde and observed by fluorescent microscopy using large field Leica DMIRB, magnification 630×. Images show the merged bright field and either the blue, yellow, or merged fluorescence. Insets show enlarged view of the phagocytized bacteria.