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Primer sequence information.
| Primer | Primers Sequence (5′→3′) | Annealing Temperature | Length (bp) |
|---|---|---|---|
| P1 | F: TTCCATTCGCATAAGTCATC R: AAAGTTGATTTGGGGATACC |
51 °C | 638 |
| P2 | F: TGTAATTGGGAATTCAAGGGGGA R: CCCATTTGGTGTGTGATAAGATGA |
58 °C | 708 |
| P3 | F: AGTCCACAGACCACAAAGCA R: TCGCAATATAAGTTTCTGATGGCTT |
58 °C | 693 |
| P4 | F: AAACCAGCAACACAAAGTC R: CATCTCAGCAAGTGCCAAG |
60 °C | 574 |
