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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: J Immunol. 2020 Mar 23;204(9):2514–2522. doi: 10.4049/jimmunol.1901508

Figure 6. IRF8 does not regulate GBPs and IRGB10 for NLRP3 inflammasome activation.

Figure 6.

(A) Immunoblot analysis of interferon regulatory factor 1 (IRF1), guanylate binding protein 2 (GBP2), and GBP5 in wild-type (WT) or Irf8–/– bone marrow-derived macrophages (BMDMs) after E. coli (multiplicity of infection [MOI], 10) or C. rodentium (MOI, 10) infection. (B) Immunoblot analysis of IRGB10 and IRF8 in WT or Irf8–/– BMDMs after E. coli (MOI, 10) infection. (C) Immunoblot analysis of phospho (p)-STAT1 and STAT1 in WT or Irf8–/– BMDMs after E. coli (MOI, 10) or C. rodentium (MOI, 10) infection. (D) Immunoblot analysis of GBP2 and GBP5 WT, Irf8–/–, or Stat1–/– BMDMs after E. coli (MOI, 10) or C. rodentium (MOI, 10) infection. (E) Immunoblot analysis of pro-caspase-1 (CASP1) (p45) and cleaved CASP1 (p20) in WT, Irf8–/–, Stat1–/–, or Casp11–/– BMDMs infected with C. rodentium (MOI, 20) for 16 h. Data are representative of three independent experiments.