Fig. 1.

IAV induces the degradation of JAK1. (a) A549 cells, 293 T cells and MDCK cells were infected or uninfected (Mock) with indicated IAV (A/mallard/Huadong/S/2005 (H5N1), A/chicken/Jiangsu/WJ-14/2015 (H7N9) and A/chicken/Taixing/10/2010 (H9N2)) at an MOI of 1 for 24 h, The protein levels of JAK1 and viral NP were analyzed by Western blotting. The protein level of β-actin was used as internal loading control. (b) 293 T cells were left uninfected or infected with IAV influenza A/mallard/Huadong/S/2005 (H5N1), which was used for the majority of the following experiments unless indicated otherwise, at an MOI of 1 for the indicated times. The levels of JAK1, viral NP and β-actin were analyzed by Western blotting. (c) 293 T cells were left uninfected or infected with IAV at the indicated MOI for 24 h. The protein levels of JAK1, viral NP and β-actin were analyzed using Western blotting