Fig. 4.

Rescuing JAK1 overexpression enhances cellular responses to type I and type II IFNs. (a) 293 T cells were transfected with control vector or plasmid encoding JAK1-His. At 24 h post-transfection, cells were infected with IAV at an MOI of 1 for an additional 24 h. The level of JAK1, viral NS1, and β-actin were detected using Western blotting. (b) 293 T cells were transfected with control vector or plasmid encoding JAK1-His. At 24 h post transfection, cells were infected with IAV at an MOI of 1. At 24 hpi, cells were left untreated (−) or treated with human IFN-α (1000 U/ml) for 1 h. The level of JAK1, pSTAT1, viral NS1, and β-actin were detected using Western blotting. (c) 293 T cells were transfected with control vector or plasmid encoding JAK1-His. At 24 h post transfection, cells were infected with IAV at an MOI of 1. At 24 hpi, cells were left untreated (−) or treated with human IFN-γ (1000 U/ml) for 1 h The level of JAK1, pSTAT1, viral NS1, and β-actin were detected using Western blotting. (d) 293 T cells were transfected with control vector or plasmid encoding JAK1-His. At 24 h post transfection, cells were left uninfected (Mock) or infected with IAV at an MOI of 1. At 24 hpi, cells were left untreated (−) or treat with human IFN-α (1000 U/ml) for 24 h. The relative mRNA levels of Mx1 were analyzed using real-time qPCR. The error bars represent the means plus standard deviations for three independent experiments. *, p ≤ 0.05. (e) 293 T cells were transfected with control vector or plasmid encoding JAK1-His. At 24 h post transfection, cells were left uninfected (Mock) or infected with IAV at an MOI of 1. At 24 hpi, cells were left untreated (−) or treat with human IFN-γ (1000 U/ml) for 6 h. The relative mRNA levels of TAP-1 were analyzed using real-time qPCR. The error bars represent the means plus standard deviations for three independent experiments. *, p ≤ 0.05