Fig. 4.

Cell-to-cell interactions and geometry arrangements of HO-8910PM cells in gel-cell clumps. HO-8910PM cells formed MCTS in three types of hydrogel matrices on days 6 (a) and days 12 (b). Gel-cell clumps of HO-8910PM cell line in RADA16-I, Matrigel and collagen I were harvested on day 6 and day 12. Immunofluorescence assay was performed to indicate cell-to-cell interactions and geometry arrangements in 3D cell culture. Red indicated F-actin and blue indicated DAPI-stained cell nuclear. Immunofluorescence images were obtained by an inverted Olympus IX71 microscope. The blue nuclear staining showed HO-8910PM cell arrangement in MCTS and the red staining surrounding blue nuclear envisioned the cell-to-cell adhesion or intercellular junction in MCTS