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. 2020 Jun 11;18:90. doi: 10.1186/s12951-020-00646-x

Fig. 5.

Fig. 5

Representative images of MCTS for cell aggregate growth patterns in one tenth scaffold concentration and cell density when cultured for 6, 9, 12, and 15 days, respectively. HO-8910PM cells formed MCTS in three types of hydrogels and maintained different cell aggregate growth patterns in 3D cell culture for 15 days. MCTS was imaged through bright-field phase contrast microscopy. Phase contrast images were some representatives for HO-8910PM cells harvested at the desired time points. Scale bar was 100 μm