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. 2020 Jun 12;17:185. doi: 10.1186/s12974-020-01857-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — In situ quantitative immunohistochemical analysis of pro-inflammatory cytokines in the NTS following feeding with 10% fructose and AZD8797 treatment. a Qualitative analysis of IL-1β and IL-6 were observed by immunohistochemical staining after administration of the CX3CR1 inhibitor AZD8797. The arrowhead indicates positive cells as a representative. The scale bar presents 50 μm. b Graphs depicting the quantitative analysis of the in situ cytokine-positive cells. The percentage was determined by counting pro-inflammatory positive cells in each hemisphere of the NTS at 200 × magnification. One-way ANOVA with Scheffe’s post-hoc was performed for statistical analysis. The values are represented as mean ± SEM. *P < 0.05 compared to control rats and ^#P < 0.05 compared to fructose-fed rats (n = 6~8 per group)