Table 1.
List of reagents used for cell culture
| Materials | Abbreviations | Company | Catalogue# |
|---|---|---|---|
| Bone morphogenetic proteins 4 | BMP4 | Peprotech | 120-05 |
| Vascular endothelial growth factor | VEGF | Peprotech | 100-20 |
| Wnt3A | Peprotech | 315-20 | |
| Activin A | Peprotech | 120-14E | |
| GSK-3β Inhibitor VIII | Calbiochem | 361549 | |
| Fibroblast growth factor alpha | FGFa | Peprotech | 100-17A |
| β-Estradiol | Sigma-Aldrich | E2257 | |
| Insulin-like growth factor 2 | IGF2 | Peprotech | 100-12 |
| Thrombopoietin | TPO | Peprotech | 300-18 |
| Heparin | StemCell Technologies | 07980 | |
| 3-Isobutyl-1-methylxanthine | IBMX | Sigma-Aldrich | I5879 |
| StemRegenin 1 | SR1 | Cellagen Technology | C7710 |
| Hydrocortisone | HC | Sigma-Aldrich | H0888 |
| Stem cell factor | SCF | Peprotech | 300-07 |
| Interleukin 3 | IL-3 | Peprotech | 200-03 |
| Erythropoietin | EPO | Stem Cell | 02625 |
| Poloxamer 188 | P188 | Sigma-Aldrich | P5556 |
| Transferrin | TF | Sigma-Aldrich | T8158 |
| Insulin | Sigma-Aldrich | I3536 | |
| Ferric nitrate | FN | Sigma-Aldrich | F8508 |
| Monothioglycerol | MTG | Sigma-Aldrich | M6145 |
| Penicillin–Streptomycin | P-S | Gibco | 15140122 |
| Y-27632 | StemCell Technologies | 72392 | |
| Stemline II hematopoietic stem cell expansion medium | Sigma-Aldrich | S0192 | |
| mTeSR1 basal medium | StemCell technologies | 85851 | |
| mTeSR1 5 × Supplement | StemCell technologies | 85852 | |
| AggreWell EB formation medium | StemCell technologies | 05893 |