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. 2020 Jun 12;13:308. doi: 10.1186/s13071-020-04169-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Lineages and genotypes of trypanosomes of the subgenus Megatrypanum inferred using ITS1 rDNA sequences. Network inferred using polymorphic ITS1 rDNA sequences of trypanosomes representative of genotypes identified in cervids (WTD, follow deer, sika deer and elk), bovids (cattle, water buffalo and antelopes), sheep keds, and sand flies. The analysis was carried out using the Neighbour-Net method with K2P parameter, and nodal support estimated with 500 bootstrap replicates. Trypanosoma trinaperronei n. sp. comprised two highly similar isolates, TCC2268 from Venezuela and WTD A3 from Texas (USA) assigned to the new genotype TthII H, which is closest to TthII C formed by Trypanosoma sp. D30 of fallow deer and TC2 of red deer from German and Croatia, respectively. Abbreviations: TCC, trypanosomatids culture collection; WTD, white-tailed deer