Table 2.
Differentially expressed proteins in response to siRNA against XIAP in MCF-7 cells
| Spot | Gene product | Score | Uniprot ID | Biological process | Molecular function | Theoretical MW (KD)/pI | On gel MW (KD)/pI | Location | Ratio* |
|---|---|---|---|---|---|---|---|---|---|
| 1 | GRP78 | 286 | P11021 | Protein metabolism | Chaperone activity | 72,402/5.07 | 77,112/4.65 | 9q33 | 0.33 |
| 2 | ENPL | 82 | P14625 | Protein metabolism | Heat shock protein activity | 92,696/4.76 | 80,516/4.55 | 12q23 | 0.27 |
| 3 | ATPB | 390 | P06576 | Metabolism, Energy pathways | Transporter activity | 56,525/5.26 | 52,427/4.65 | 12q13 | 0.66 |
| 4 | GDIB | 103 | P50395 | Transport | Auxiliary transport protein activity | 51,087/6.11 | 46,950/6.1 | 10p15 | 2.3 |
| 5 | GRP75 | 274 | P38646 | Protein metabolism | Chaperone activity | 73,920/5.87 | 71,587/5.9 | 5q31 | 0.51 |
| 6 | G6PD | 257 | P11413 | Metabolism, Energy pathways | Catalytic activity | 59,675/6.39 | 55,639/6.1 | Xq28 | 0.49 |
| 7 | ENO1 | 735 | P06733 | Metabolism, Energy pathways | Catalytic activity | 47,481/7.01 | 45,701/6.3 | 1p36 | 3.3 |
| 8 | ALBU | 73 | P02768 | Transport | Transporter activity | 71,317/5.92 | 66,546/5.4 | 4q13 | 0.51 |
| 9 | PRDX2 | 401 | P32119 | Metabolism; Energy pathways | Peroxidase activity | 22,049/5.66 | 22,089/5.4 | 19p13 | 0.39 |
| 10 | TCPE | 122 | P48643 | Protein metabolism | Chaperone activity | 60,089/5.45 | 64,545/5.3 | 5p15 | 0.64 |
| 11 | FKBP4 | 102 | Q02790 | Metabolism, Energy pathways | Isomerase activity | 52,057/5.35 | 50,535/5.2 | 12p13 | 0.43 |
| 12 | HS71A | 234 | P0DMV8 | Protein metabolism | Chaperone activity | 70,294/5.48 | 71,325/5.3 | 6p21 | 0.68 |
| 13 | HS71B | 234 | P0DMV9 | Protein metabolism | Chaperone activity | 70,294/5.48 | 81,261/5.3 | 6p21 | 0.41 |
| 14 | HSP7C | 526 | P11142 | Protein metabolism | Heat shock protein activity | 71,082/5.37 | 71,261/5.4 | 11q24.1 | 0.68 |
| 15 | HS90A | 32 | P07900 | Protein metabolism | Chaperone activity | 85,006/4.94 | 91,559/4.7 | 14q32 | 0.1 |
| 16 | NPM | 109 | P06748 | Protein metabolism | Chaperone activity | 32,726/4.64 | 38,294/4.3 | 5q35 | 0.65 |
| 17 | CRK | 103 | P46108 | Cell communication, Signal transduction | Receptor signaling complex scaffold activity | 338,675.38 | 29,195/5.0 | 17p13 | 0.03 |
| 18 | PA2G4 | 70 | Q9UQ80 | Regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolism | Transcription regulator activity | 44,101/6.13 | 70,963/6.3 | 12q13 | 0.63 |
| 19 | SERA | 140 | O43175 | Metabolism; Energy pathways | Catalytic activity | 57,356/6.29 | 77,142/5.4 | 1p12 | 2.72 |
| 20 | TBA1B | 840 | P68363 | Cell growth and/or maintenance | Structural molecule activity | 50,804/4.94 | 58,606/4.6 | 12q13 | 0.65 |
| 21 | SAM50 | 36 | Q9Y512 | unknown | unknown | 52,342/6.44 | 17,144/5.4 | 22q13 | 0.07 |
| 22 | KPYM | 118 | P14618 | Energy pathways, metabolism | Kinase activity | 58,470/7.96 | 60,963/6.3 | 15q23 | 0.63 |
| 23 | PRDX6 | 112 | P30041 | Metabolism, Energy pathways | Peroxidase activity | 25,133/6.00 | 27,314/5.9 | 1q25 | 0.06 |
| 24 | HSPB1 | 414 | P04792 | Protein metabolism | Chaperone activity | 22,826/5.98 | 20,312/5.7 | 7q11 | 0.41 |
| 25 | CH60 | 623 | P10809 | Protein metabolism | Heat shock protein activity | 61,187/5.70 | 68,904/5.4 | 2q33 | 2.3 |
| 26 | TCPZ | 93 | P40227 | Protein metabolism | Chaperone activity | 58,444/6.23 | 60,904/6.0 | 7p11 | 0.44 |
| 27 | TBB5 | 504 | P07437 | Cell growth and/or maintenance | Structural constituent of cytoskeleton | 50,095/4.78 | 54,486/4.8 | 6p21 | 0.75 |
| 28 | IF5A1 | 90 | P63241 | Protein metabolism | Translation factor activity, nucleic acid binding | 17,049/5.08 | 1646/4.8 | 17p13 | 0.6 |
| 29 | EF1G | 148 | P26641 | Protein metabolism | Translation regulator activity | 50,429/6.25 | 53,625/5.9 | 11q12 | 0.5 |
| 30 | PDIA1 | 635 | P07237 | Protein metabolism | Isomerase activity | 57,480/4.76 | 60,784/4.5 | 17q25 | 0.83 |
The product of CH60, ENO1, and GDIB genes were increasingly expressed (> twofold)