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. 2020 Jun 12;17:183. doi: 10.1186/s12974-020-01864-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Western blot of whole protein extracts of dorsal root ganglia (DRG) nuclei and cytosol fractions of rats with hindpaw inflammation versus control (Ctrl) with anti-mineralocorticoid receptor (MR), Poly(ADP-ribose)polymerase (PARP), or ß-actin antibody. a, b Western blots of the nuclear and cytosol fractions were verified by the detection of the nuclear component Poly(ADP-ribose)polymerase (PARP) (110 kDa) and the cytosol component ß-actin (43 kDa), respectively. In both the nuclear and cytosol fractions MR (107 kDa), specific protein was detected under inflamed and control conditions. However, nuclear fractions from DRG innervating inflamed hindpaws showed a significant 8-fold increase in the density of the MR protein compared to control DRG (P < 0.05, two-tailed independent Student’s t test; n = 6). c, d Confocal microscopy showing increasing overlap of MR-immunorecativity (red fluorescence) with nuclear DAPI (blue fluorescence) staining of DRG of FCA-treated rats versus controls