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. 2020 Jun 11;20:184. doi: 10.1186/s12906-020-02970-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Densitogram obtained from Standard- ursolic and oleanolic acid (1 a) using n-hexane: ethyl acetate: acetone (16.4: 3.6: 0.2 v/v) mobile phase; formulation samples containing ursolic and oleanolic acid (1 b); Standard-mangiferin (2 a); formulation samples containing mangiferin (2 b) using ethyl acetate: glacial acetic acid: formic acid: water (20: 2.2: 2.2: 5.2 v/v) mobile phase; Standard- gallic acid (3 a); Standard- quercetin (3 b); Standard- curcumin (3 c) using toluene: ethyl acetate: formic acid (13.5: 9: 0.6 v/v) mobile phase; and formulation samples contains gallic acid, quercetin and curcumin (3 d). HPTLC fingerprinting (1c; 2c; 3e) showing track 1; 2; 3–5: STANDARD-oleanolic and ursolic acid; mangiferin; gallic acid; quercetin and curcumin respectively where tracks M1, M2 and M3 signifying marker compounds in three different commercial formulations (MC1, MC2 and MC3)