Table 2.
Analytical characteristics of the method validation
| Sr. no. | Parameters | Mangiferin (M) | Ursolic acid (UA) | Oleanolic acid (OA) | Gallic acid (GA) | Quercetin (Q) | Curcumin (C) |
|---|---|---|---|---|---|---|---|
| Precision and accuracy | |||||||
| 1 | Intra-day RSD (%), n = 5 | 0.437 | 0.357 | 0.487 | 0.67 | 0.56 | 0.45 |
| 2 | Inter-day RSD (%),n = 5 (day-1/day-2/day-3) | 0.437/0.495/0.512 | 0.353/0.425/0.532 | 0.484/0.498/0.514 | 0.67/0.68/0.71 | 0.56/0.59/0.63 | 0.45/0.48/0.49 |
| Recovery | |||||||
| 3 | Amount of standard in MC samples (μg mg−1) containing highest bioactive compounds | 44.6 | 8.1 | 19.2 | 20.1 | 10.3 | 6.1 |
| 4 | Amount of standards added in MC sample (μg mg− 1) | 20/40/60 | 4/8/12 | 10/20/30 | 10/20/30 | 5/10/15 | 3/6/9 |
| 5 | Amount of standard found (μg mg−1) | 64.36/84.4/104.26 | 12.1/16.2/20.11 | 29.21/39.19/49.12 | 30.12/40.13/50.03 | 15.29/20.28/25.33 | 9.1/12.21/15.09 |
| 6 | Recovery (%) | 99.62/99.76/99.67 (99.81) | 100/100.62/100.04 | 100.03/99.97/99.83 | 100.06/100.07/99.86 | 99.93/100.89/99.9/100.11 | 100/100.9/99.93 |