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. 2020 Jun 11;20:155. doi: 10.1186/s12866-020-01818-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Flow cytometric DIBAC₄ (3) (FITC-A)/PI (PC5.5A) dot plots representing Escherichia coli viability through membrane potential and membrane integrity of a ciprofloxacin (CIP)-susceptible (a) and CIP-resistant (b) Escherichia coli strain after treatment with three increasing CIP concentrations either with or without benzalkonium chloride (BKC) exposure. The green region corresponds to the live subpopulation with an intact membrane and normal membrane potential, the blue region corresponds to the intermediate subpopulation with different degrees of compromised membranes and loss of membrane potential, the red region corresponds to the dead subpopulation with irreversibly damaged membrane and complete loss of membrane potential. Percentages are the mean of triplicate experiments